Quantitative PCR for tracking the megaplasmid-borne biodegradation potential of a model sphingomonad.
نویسندگان
چکیده
We developed a quantitative PCR method for tracking the dxnA1 gene, the initial, megaplasmid-borne gene in Sphingomonas wittichii RW1's dibenzo-p-dioxin degradation pathway. We used this method on complex environmental samples and report on growth of S. wittichii RW1 in landfill leachate, thus furnishing a novel tool for monitoring megaplasmid-borne, dioxygenase-encoding genes.
منابع مشابه
Potentials of Evolving Linear Models in Tracking Control Design for Nonlinear Variable Structure Systems
Evolving models have found applications in many real world systems. In this paper, potentials of the Evolving Linear Models (ELMs) in tracking control design for nonlinear variable structure systems are introduced. At first, an ELM is introduced as a dynamic single input, single output (SISO) linear model whose parameters as well as dynamic orders of input and output signals can change through ...
متن کاملAssay of Tgf-β And B-Fgf on the Potential of Peripheral Blood-Borne Stem Cells and Bone Marrow-Derived Stem Cells in Wound Healing in a Murine Model
Purpose: Effects of TGF-b and b-FGF on the Potential of Peripheral Blood-Borne Stem Cells and Bone Marrow-Derived Stem Cells In Wound Healing in a Murine Model.Materials and Methods: Peripheral blood mesenchymal stem cells (PBMSCs) and bone marrow stem cells (BMSCs) cultured in media with transforming growth factor-beta (TGF-b) and basic fibroblast growth factor (b-FGF). Stem cells labeled with...
متن کاملThe study of impranil (DLN) polymer biodegradation by fungus Sarocladium kiliense
Impranil DLN is a class of plastics belonging to the polyurethane family with high application in textile industries. The aim of this study was to evaluate the potential of native strain to degrade impranil DLN. In this study, yeast strains were isolated from different areas and purified in minimal medium containing 1% impranil. Isolate NS-10 was selected as the superior strain capable of degra...
متن کاملPotential Use of DNA Aptamer-Magnetic Bead Separation-PCR Assay for Salmonella Detection in Food
Background: Salmonella is one of the most common food-borne pathogens that can cause illness. In this study, the sensitivity and the specificity of Aptamer-Magnetic bead Separation-Polymerase Chain Reaction (AMS-PCR) method were determined for Salmonella spp. detection. Methods: Different concentrations of Salmonella enterica were mixed with streptavidin-magnetic beads coated with biotinylated...
متن کاملبررسی مدل تجزیه زیستی فنانترین در خاک های آلوده توسط اسینتوباکترSP
Backgrounds and Objectives: Polycyclic aromatic hydrocarbons (PAHs) are a group of hazardous pollutants which have carcinogenic and mutagenic properties and accumulated in environment by different actions, therefore treatment of them is important. Biological treatments are simple and cheep technologies. This technology was recommended as a cost- effective method for treatment of these pollutant...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 78 12 شماره
صفحات -
تاریخ انتشار 2012